Exploring the RNA world, discovering new modes of regulation and how they are used throughout biology from yeast to stem cells.



Ferdous AS, Costa Dos Santos SJ, Kanzler CR, Shin H, Carrick BH, Crittenden SL, Wickens M, Kimble J. (2023) The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells. Development. 150(9):dev201705. doi: 10.1242/dev.201705. [PubMed] [PDF]
Updated article from bioRxiv. (2023) doi: 10.1101/2023.02.15.528708. [PubMed]


Zhou W, Melamed D, Banyai G, Meyer C, Tuschl T, Wickens M, Cao J, Fields S. (2021) Expanding the binding specificity for RNA recognition by a PUF domain. Nat Commun. 12(1):5107. doi: 10.1038/s41467-021-25433-6 [PubMed] [PDF] Publisher correction (2022) [PubMed]

Shukla A, Yan J, Pagano DJ, Dodson AE, Fei Y, Gorham J, Seidman JG, Wickens M, Kennedy S. (2021) Author Correction: poly(UG)-tailed RNAs in genome protection and epigenetic inheritance. Nature 592(7856):E27. doi: 10.1038/s41586-021-03364-y. [PubMed] Original article: doi: 10.1038/s41586-020-2323-8. [PubMed]

Aoki ST, Lynch TR, Crittenden SL, Bingman CA, Wickens M, Kimble J. (2021) C. elegans germ granules require both assembly and localized regulators for mRNA repression. Nat Commun. 12(1):996. doi: 10.1038/s41467-021-21278-1. [PubMed] [PDF]


Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M. (2020) Records of RNA locations in living yeast revealed through covalent marks. Proc Natl Acad Sci USA. 117(38):23539-23547. doi: 10.1073/pnas.1921408117. [PubMed] [PDF]

Shukla A, Yan J, Pagano DJ, Dodson AE, Fei Y, Gorham J, Seidman JG, Wickens M, Kennedy S. (2020) poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance. Nature. 582(7811):283-288. doi: 10.1038/s41586-020-2323-8. [PubMed] [PDF]

Haupt KA, Law KT, Enright AL, Kanzler CR, Shin H, Wickens M, Kimble J. (2020) A PUF Hub Drives Self-Renewal in Caenorhabditis elegans Germline Stem Cells. Genetics. 214(1), 147-161. doi: 10.1534/genetics.119.302772. [PubMed] [PDF]

This is an accordion element with a series of buttons that open and close related content panels.

2015 - 2019


Haupt KA, Enright AL, Ferdous AS, Kershner AM, Shin H, Wickens M, Kimble J. (2019) The molecular basis of LST-1 self-renewal activity and its control of stem cell pool size. Development. 146(20). doi: 10.1242/dev.181644. [PubMed] [PDF]

Qiu C, Dutcher RC, Porter DF, Arava Y, Wickens M, Hall TMT. (2019) Distinct RNA-binding modules in a single PUF protein cooperate to determine RNA specificity. Nucleic Acids Res. 47(16), 8770–8784. doi: 10.1093/nar/gkz583. [PubMed] [PDF]

Preston, M.A., Porter, D.F., Chen, F., Buter, N., Lapointe, C.P., Keles, S., Kimble, J., and Wickens, M. (2019) Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase. Nature Methods. 16, 437-445. doi: 10.1038/s41592-019-0370-6. [PubMed] [PDF]

Bhat, V.D., McCann, K.L., Wang, Y., Fonseca, D.R., Shukla, T., Alexander, J.C., Qiu, C., Wickens, M., Lo, T.W., Hall, T.M.T., and Campbell, Z.T. (2019) Engineering a conserved RNA regulatory protein repurposes its biological function in vivo. eLIFE. 2019;8:e43788. doi: 10.7554/eLife.43788. [PDF]


Porter DF, Prasad A, Carrick BH, Kroll-Conner P, Wickens M, Kimble J. (2018) Toward Identifying Subnetworks from FBF Binding Landscapes in Caenorhabditis Spermatogenic or Oogenic Germlines. G3: Genes, Genomes, Genetics. 9(1), 153-165. doi: 10.1534/g3.118.200300. [PubMed] [PDF]

Aoki ST, Porter DF, Prasad A, Wickens M, Bingman CA, Kimble J. (2018) An RNA-Binding Multimer Specifies Nematode Sperm Fate. Cell Rep. 23(13), 3769-3775. doi: 10.1016/j.celrep.2018.05.095. [PubMed] [PDF]

Lapointe CP, Stefely JA, Jochem A, Hutchins PD, Wilson GM, Kwiecien NW, Coon JJ, Wickens M, Pagliarini DJ. (2018) Multi-omics Reveal Specific Targets of the RNA-Binding Protein Puf3p and Its Orchestration of Mitochondrial Biogenesis. Cell Syst. 6, 125-135.e6. doi: 10.1016/j.cels.2017.11.012. [PubMed] [PDF]


Shin H, Haupt KA, Kershner AM, Kroll-Conner P, Wickens M, Kimble J. (2017) SYGL-1 and LST-1 link niche signaling to PUF RNA repression for stem cell maintenance in Caenorhabditis elegans. PLoS Genet. 13(12):e1007121.
doi: 10.1371/journal.pgen.1007121. [PubMed] [PDF]

Wilinski D, Buter N, Klocko AD, Lapointe CP, Selker EU, Gasch AP, Wickens M. (2017) Reply to Hogan: Direct evidence of RNA-protein interactions and rewiring. Proc Natl Acad Sci U S A. 114, E10854-E10855. doi: 10.1073/pnas.1717585114. [PubMed] [PDF]

Lapointe CP, Wickens M. (2017) RNA Tagging: Preparation of High-Throughput Sequencing Libraries. Methods Mol Biol. 1649, 455-471. doi: 10.1007/978-1-4939-7213-5_30. [PubMed] [PDF]

Lapointe CP, Preston MA, Wilinski D, Saunders HAJ, Campbell ZT, Wickens M. (2017) Architecture and dynamics of overlapped RNA regulatory networks. RNA. 23, 1636-1647. doi: 10.1261/rna.062687.117. [PubMed] [PDF]

Wilinski, D., Buter, N., Lapointe, C., Klocko, A.D., Saunders, H., Selker, E., Gasch, A. and Wickens, M. (2017) Recurrent rewiring in RNA regulatory networks. Proc Natl Acad Sci USA. 114, E2816–E2825, doi: 10.1073/pnas.1617777114. [PubMed] [PDF]


Prasad, A., Porter, D.F., Kross-Conner, P.L., Mohanty, I., Ryan, A.R., Crittenden, S.L., Wickens, M. and Kimble, J. (2016) PUF binding landscape in metazoan germ cells. RNA. 22, 1026-1043. [PubMed] [PDF]

Aoki, S.T., Kershner, A.M., Bingman, C.A., Wickens, M. and Kimble, J. (2016). PGL germ granule assembly protein is a base-specific, single-stranded RNase. Proc Natl Acad Sci USA. 113, 1279-1284. [PubMed] [PDF]


Wickens, M. (2015). Lessons from the RNA World: humility and hubris. RNA, 21, 482. [PubMed] [PDF]

Porter, DF, Koh, YY, VanVeller, B, Raines, RT, and Wickens, M. (2015). Target selection by natural and redesigned PUF proteins. Proc Natl Acad Sci USA. 112, 15868-15873. [PubMed] [PDF]

Lapointe, CP, Wilinski, D, and Wickens, M. (2015). Protein-RNA networks revealed through covalent RNA Marks. Nature Methods. 12, 1163-1170. [PubMed] [PDF]

Wilinski, D, Chen, Q, Lapointe, CP, Nevil, M, Campbell, ZT, Hall, TMT, and Wickens, M. (2015). RNA regulatory networks diversified through curvature of the PUF protein scaffold. Nature Communications. 6. 8213. [PubMed] [PDF]

Waghray, S, Williams, C, Coon, JJ, and Wickens, M. (2015). Xenopus CAF1 requires NOT1 mediated interaction with 4E-T to repress translation in vivo. RNA. 21, 1335-1345. [PubMed] [PDF]

Campbell, Z and Wickens M. (2015). Probing RNA-protein networks: Biochemistry meets genomics. Trends in Biochemical Sciences, 40, 157-164. [PubMed] [PDF]

2010 - 2014


Campbell, ZT, Valley, CT and Wickens, M. (2014). A protein-RNA specificity code enables targeted activation of an endogenous human transcript. Nature Structural & Molecular Biology. 21, 732-738. [PubMed] [PDF]

Koh YY and Wickens M. (2014). Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system. Methods Enzymology (Elsevier), Vol. 539, Chapter 11, 195-214. [PubMed] [PDF]

Koh YY and Wickens M. (2014). Dissecting a known RNA-protein interaction using a yeast three-hybrid system. Methods Enzymology. (Elsevier), Vol. 539, Chapter 10, 183-193. [PubMed] [PDF]

Koh YY and Wickens M. (2014). Determining the RNA specificity and targets of an RNA-binding protein using a yeast three-hybrid system. Methods Enzymology. (Elsevier), Vol. 539, Chapter 9, 163-181. [PubMed] [PDF]

Ellery, PER, Maroney, SA, Martinez, ND, Wickens, MP, and Mast, AE. (2014). Translation of Human Tissue Factor Pathway Inhibitor-Beta mRNA is Controlled by Alternative Splicing within the 5’UTR. Arteriosclerosis, Thromb, and Vasc Biol., 34, 197-195. [PubMed] [PDF]


Zhang, Y, Cooke, A, Park, S, Dewey, CN, Wickens, M, and Sheets, MD. (2013). Bicaudal-C spatially controls translation of vertebrate maternal mRNAs. RNA. 19, 1575-1582. [PubMed] [PDF]

Lapointe, CP, and Wickens, M. (2013). The Nucleic Acid Binding Domain and Translational Repression Activity of a Xenopus Terminal Uridylyl Transferase. J Biol Chem. 288, 20723-20733. [PubMed] [PDF]

LeGendre, JB, Campbell, ZT, Kroll-Conner, P, Anderson, P, Kimble, J, and Wickens, M. (2013). RNA targets and specificity of Staufen, a double-stranded RNA-binding protein in C. elegans. J Biol Chem. 288, 2532-2545. [PubMed] [PDF]

Wu, J, Campbell, ZT, Menichelli, E, Wickens, M, and Williamson, JR. (2013). A Protein-Protein interaction Platform Involved in Recruitment of GLD-3 to the FBF-fem-3 mRNA Complex. J Mol Biol. 425, 738-754. [PubMed] [PDF]

Menichelli, E, Wu, J, Campbell, ZT, Wickens, M, and Williamson, JR. (2013). Biochemical Characterization of the Caenorhabditis elegans FBF-CPB-1 Translational Regulation Complex Identifies Conserved Protein Interaction Hotspots. J Mol Biol. 425, 725-737. [PubMed] [PDF]


Campbell, ZT, Menichelli, E, Friend, K, Kimble, J, Williamson, JR and Wickens, M. (2012). Identification of a conserved interface between PUF and CPEB proteins. J Biol Chem. 287, 18854-18862. [PubMed] [PDF]

Campbell, ZT, Bhimsaria, D, Valley, CT, Rodriguez-Martinez, J., Menichelli, E, Williamson, JR, Ansari, AZ and Wickens, M. (2012). Cooperativity in RNA-protein interactions: Global analysis of RNA binding specificity. Cell Rep. 1, 570-581. [PubMed] [PDF]

Valley, C, Porter, D, Campbell, Z and Wickens, M. (2012). Patterns and plasticity in PUF-RNA interactions: embedded information in binding sites. Proc Natl Acad Sci USA. 109, 6054-6059. [PubMed] [PDF]

Liu Q, Stumpf C, Wickens M, Haag E. (2012). Context-dependent function of a conserved translational regulatory module. Development. 139, 1509-1521. [PubMed] [PDF]

Gerstner, JR, Vanderheyden, WM, LaVaute, T, Westmark, CJ, Rouhana, L, Pack, AI, Wickens, M, and Landry, CF. (2012). Time of day regulates subcellular trafficking, tripartite synaptic localization, and polyadenylaton of the astrocytic fabp7 mRNA. J Neurosci., 32, 1383-1394. [PDF]

Friend, K, Campbell, ZT, Cooke, A, Kroll-Conner, P, Wickens, MP, and Kimble, J. (2012). A conserved PUF-Ago-eEF1A complex attenuates translation elongation. Nat Struct Mol Biol. 19, 176-183. [PubMed] [PDF]

Qiu, C, Kershner, A, Wang, Y, Holley, CP, Wilinski, D, Keles, S, Kimble, J, Wickens, M, and Hall, TM. (2012). Divergence of Pumilio/fem-3 mRNA binding factor (PUF) protein specificity through variations in an RNA-binding pocket. J Biol Chem. 287, 6949-6957. [PubMed] [PDF]


Koh, Y, Wang, Y, Qiu, C, Opperman, L, Gross, L, Hall, TM, Wickens, M. (2011) Stacking interactions in PUF-RNA complexes. RNA, 17, 718-727. [PubMed] [PDF]

Yosefzon Y, Koh YY, Chritton JJ, Lande A, Leibovich L, Barziv L, Petzold C, Yakhini Z, Mandel-Gutfreund Y, Wickens M, Arava Y. (2011) Divergent RNA binding specificity of yeast Puf2p. RNA, 17, 1479-1488. [PubMed] [PDF]

Chritton, JJ, Wickens, M. (2011) A role for the Poly(A) binding protein Pab1p-mediated repression. J Biol Chem, 286, 33268-33278. [PubMed] [PDF]

Cooke, A, Prigge, A, Opperman, L, Wickens, M. (2011) Targeted translational regulation using the PUF scaffold. Proc Natl Acad Sci USA, 108, 15870-15875. [PubMed] [PDF]


Cooke, A, Prigg, A, Wickens, M. (2010) Translational repression by deadenylases. J Biol Chem, 16, 28506-28513. [PubMed] [PDF]

Chritton, JJ, Wickens, M. (2010) Translational repression by PUF proteins in vitro. RNA, 16 6):1217-25. [PubMed] [PDF]

2005 - 2009


Zhu, D, Stumpf, CR, Krahn, JM, Wickens, M, Hall, TM. (2009) A 5′ cytosine binding pocket in Puf3p specifies regulation of mitochondrial mRNAs. ProcNatlAcadSci USA, 106(48):20192-7. [PubMed]  [PDF]

Wang, Y, Opperman, L, Wickens, M, Hall, TM. (2009) Structual basis for specific recognition of multiple mRNA targets by a PUF regulatory protein. ProcNatlAcadSci USA, 106(48):20186-91. [PubMed] [PDF]

Koh, Y, Opperman, L, Stumpf, C, Mandan, A, Keles, S, Wickens, M. (2009) A single C. elegans Puf protein binds RNA in multiple modes. RNA, 15 (6):1090-9. [PubMed] [PDF]

Suh, N, Crittenden, SL, Goldstrohm, A, Hook, B, Thompson, B, Wickens, M, Kimble, J. (2009) FBF and its dual control of gld-1 expression in the Caenorhabditis elegans germline. Genetics, 181 (4):1245-60. [PubMed] [PDF]


Stumpf, C, Opperman, L, Wickens, M. (2008) Analysis of RNA–Protein Interactions Using a Yeast Three-Hybrid System. Methods in Enzymology, Vol 449. [PubMed] [PDF]

Goldstrohm, A, Hook, B, Wickens, M. (2008) Regulated Deadenylation In Vitro. Methods in Enzymology, Volume 448, 77-106. [PubMed] [PDF]

Kwak, JE, Drier, E, Barbee, SA, Ramaswami, M, Yin, JC, Wickens, M. (2008) GLD2 poly(A) polymerase is required for long-term memory. Proc Natl Acad Sci U S A, 105(38):14644-9. [PubMed] [PDF]

Stumpf, CR, Kimble, J, Wickens, M. (2008) A Caenorhabditis elegans PUF protein family with distinct RNA binding specificity. RNA, 14(8):1550-7. [PubMed] [PDF]

Benoit, P, Papin, C, Kwak, JE, Wickens, M, Simonelig, M. (2008) PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. Development, 135(11):1969-79. [PubMed] [PDF]

Goldstrohm, AC, Wickens, M. (2008) Multifunctional deadenylase complexes diversify mRNA control. Nat Rev Mol Cell Biol, 9(4):337-44. [PubMed] [PDF]

Wickens, M, Kwak, JE. (2008) A tail tale for U. Science, 319(5868):1344-5. [PubMed] [PDF]


Lee, MH, Hook, B, Pan, G, Kershner, AM, Merritt, C, Seydoux, G, Thomson, JA, Wickens, M, Kimble, J. (2007) Conserved regulation of MAP kinase expression by PUF RNA-binding proteins. PLoS Genet, 28;3 (12). [PubMed] [PDF]

Coller, J, Wickens, M. (2007) Tethered function assays: and adaptable approach to study RNA regulatory proteins. Methods enzymol, 429:299-321. [PubMed] [PDF]

Kwak, JE, Wickens, M. (2007) A family of poly(U) polymerases. RNA, 13(6):680-7. [PubMed] [PDF]

Hook, BA, Goldstrohm, AC, Seay, DJ, Wickens, M. (2007) Two yeast PUF proteins negatively regulate a single mRNA. J Biol Chem, 25;282(21):15430-8. [PubMed] [PDF]

Rouhana, L, Wickens, M. (2007) Autoregulation of GLD-2 cytoplasmic poly (A) polymerase. RNA, 13(2):188-99. [PubMed] [PDF]

Goldstrohm, AC, Seay, DJ, Hook, BA, Wickens, M. (2007) PUF protein-mediated is catalyzed by Ccr4p.  J Biol Chem,  5;282(1):109-14. [PubMed] [PDF]


Seay, D, Hook, B, Evans, K, Wickens, M. (2006) A three-hybrid screen identifies mRNAs controlled by a regulatory protein. RNA, 12(8):1594-600. [PubMed] [PDF]

Lee, M-H, Hook, B, Lamont, LB, Wickens, M, Kimble J. (2006) LIP-1 phosphatase controls the extent of germline proliferation in Caenorhabditis elegans. EMBO J, 25, 88-96. [PubMed] [PDF]

Goldstrohm, AC, Hook, BA, Seay, DJ, Wickens, M. (2006) PUF proteins bind Pop2p to regulate messenger RNAs. Nature Structural & Molecular Biology. 13(6):533-539. [PubMed] [PDF]

Suh N, Jedamzik B, Eckmann CR, Wickens M and Kimble J. (2006). The GLD-2 poly(A) polymerase activates gld-1 mRNA in the Caenorhabditis elegans germ line. Proc Natl Acad Sci USA. 103, 15108-15112. [PubMed] [PDF]


nature structural & molecular biology cover relating to Opperman article
Stem cells proliferate at one end of the C. elegans germline, and sperm differentiate at the other. FBF, a pumilio-like protein, promotes proliferation in the germline by repressing gld-1 mRNA. Opperman et al. show that FBF requires a single ‘extra’ nucleotide in its RNA targets, and so specifically binds gld-1 and not other RNAs. Blue is DAPI, green is a sperm marker. Images provided by S. Crittenden and J. Kimble.

Opperman, L, Hook, B, DeFino, M, Bernstein, D.S., Wickens, M. (2005) A single spacer nucleotide determines the specificities of two mRNA regulatory proteins. Nature Structural & Molecular Biology. 12, 945-951. [PubMed] [PDF] Cover right

Rouhana, L, Wang, L, Buter, N, Kwak, JE, Schiltz, CA, Gonzalez, T, Kelley, AE, Landry, CF, Wickens, M. (2005) Vertebrate GLD2 poly(A) polymerases in the germline and the brain. RNA, 11, 1117-1130. [PubMed] [PDF]

Thompson, BE, Bernstein, D, Bachorik, J, Petcherski, A, Wickens, M, Kimble, J. (2005) Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB. Development, 132, 3471-3481. [PubMed] [PDF]

Bernstein, D, Hook, B, Hajarnavis, A, Opperman, L, Wickens, M. (2005) Binding specificity and mRNA targets of a C. elegans PUF protein, FBF-1. RNA, 11:447–458. [PubMed] [PDF]

Hook, B, Bernstein, D., Zhang, B, Wickens, M. (2005) RNA-protein interactions in the yeast three-hybrid system: affinity, sensitivity and enhanced library screening. RNA, 11, 227-233. [PubMed] [PDF]

2000 - 2004


Lamont, LB, Crittenden, SL, Bernstein, D, Wickens, M, Kimble, J. (2004)  FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans germline. Dev. Cell. 7, 697-707. [PubMed] [PDF]

Wickens, M. Gonzalez, TN. (2004) Knives, Accomplices,and RNA. Science Nov19:306, 1299-1300. [PubMed] [PDF]

Wang, L, Kimble, J, Wickens, M. (2004) Tissue-specific modification of gld-2 mRNA in C. elegans: likely C-to-U editing. RNA. Sep;10(9):1444-8. [PubMed] [PDF]

Kwak, JE, Wang, L, Ballantyne, S, Kimble, J, Wickens, M. (2004) Mammalian GLD-2 homologs are poly(A) polymerases. Proc Natl Acad Sci U S A. Mar 30;101(13):4407-12. [PubMed] [PDF]

Butcher, S, Wickens, M. (2004) Star-studded circuitry. Nature Structural and Molecular Biology 11, 2-3. [PubMed] [PDF]


Wickens, M, Goldstrohm, A. (2003) A place to die, a place to sleep. Science 300, 753-755. [PubMed] [PDF]


Eckmann, CR, Kraemer, B, Wickens, M, Kimble, J. (2002) GLD-3, a bicaudal-C homolog that inhibits FBF to control germline sex determination in C. elegans. Dev Cell. Nov;3 (5):697-710. [PubMed] [PDF]

Crittenden, SL, Bernstein, DS, Bachorik, JL, Thompson, BE, Gallegos, M, Petcherski, AG, Moulder, G, Barstead, R, Wickens, M, Kimble, J. (2002) A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans. Nature 417, 660-664. [PubMed] [PDF]

Wang, L, Eckmann, CR, Kadyk, LC, Wickens, M, Kimble J. (2002) A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans. Nature 419, 312-316. [PubMed] [PDF]

Coller, J, Wickens, M. (2002) Tethered function assays using 3(‘) untranslated regions. Methods 2, 142-50. [PubMed] [PDF]

Bernstein, B, Buter, N, Stumpf, C, Wickens, M. (2002) Analyzing mRNA-protein complexes using a yeast three-hybrid system: methods and applications. Methods 2, 123-41. [PubMed] [PDF]

Wickens, M, Bernstein, DS, Kimble, J, Parker, R. (2002) A PUF family portrait: 3’UTR regulation as a way of life. Trends in Genetics 18, 150-156. [PubMed] [PDF] [Supplement]


Dickson, K, Thompson, SR, Gray, NK, Wickens, M. (2001) Poly(A) polmerase and the regulation of cytoplasmic polyadenylation. J Biol Chem 276, 41810-41816.  [PubMed] [PDF]

Wickens, M, Bernstein, D, Crittenden, S, Luitjens, C, Kimble, J. (2001) PUF proteins and 3’UTR regulation in the C. elegans germline. In Cold Spring Harbor Symposium Volume, Cold Spring Harbor Laboratory Press, Woodbury, NY.


Luitjens, C, Gallegos, M, Kraemer, B, Kimble, J, Wickens, M. (2000) CEPB proteins control two key steps in spermatogenesis in C. elegans. Genes Dev. 14, 2596-2609. [PubMed] [PDF]

Wickens, M, Goodwin, EB, Kimble, J,  Strickland, S, Hentze, M. (2000) Translational Control in Developmental Decisions. In Translational Control Second Edition. (Michael Mathews, ed.) Cold Spring Harbor Press, New York, pp. 295-370. [PDF]

Kraemer, B, Zhang, B, SenGupta, D,  Fields, S, Wickens, M. (2000) A yeast three-hybrid system to detect and analyze interactions between RNA and protein. In RNA-Ligand Interactions. (Daniel W. Celander and John N. Abelson, eds.) of the Methods in Enzymology Series. Academic Press, New York. Vol. 328: Part III.

Gray, NK, Coller, JM, Dickson, K, Wickens, M. (2000) Multiple portions of poly(A)-binding protein stimulate translation in vivo. EMBO J. 19, pp. 4723-4733. [PubMed] [PDF]

Barkoff, AF, Dickson, KS, Gray, NK, Wickens, M. (2000) Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation. Developmental Biology 220, 97-109. [PubMed] [PDF]

Thompson, SR, Goodwin, EB, Wickens, M. (2000) Rapid deadenylation and poly(A)-dependent translational repression mediated by the C. elegans tra-2 3’UTR in Xenopus embryos. Mol & Cell Biol. 20, 2129-2137. [PubMed] [PDF]

1995 - 1999


Zhang, B, Kraemer, B, SenGupta, D, Fields, S, Wickens, M. (1999) A three-hybrid system to detect and analyze RNA-protein interactions in vivo. In Expression of Recombinant Genes in Eukaryotic Systems. (J. Glorioso and M. Schmidt, eds.) of the Methods in Enzymology Series. Academic Press, New York. Vol. 306, pp. 93-113.

Kraemer, B, Crittenden. S, Gallegos, M, Moulder, G, Barstead, R, Kimble, J, Wickens, M. (1999) NANOS-3 and FBF proteins physically interact to control the sperm/oocyte switch in C. elegans. Current Biology 9, 1009-1018. [PubMed] [PDF]

Dickson, K, Bilger, A, Ballantyne, S, Wickens, M. (1999) CPSF in X. laevis oocytes: A cytoplasmic factor involved in regulated polyadenylation. Mol. & Cell. Biol. 19, 5707-5717. [PubMed] [PDF]

SenGupta, DJ, Wickens, M, Fields, S. (1999) Identification of RNAs that bind to a specific protein using the yeast three-hybrid system. RNA 5, 596-601. [PubMed] [PDF]

Lee, E-g, Yeo, A, Kraemer, B, Wickens, Linial, ML. (1999) The Gag domains required for avian retroviral RNA encapsidation determined by using two independent assays. Journal of Virology 73, 6282-6292. [PubMed] [PDF]


Gray, N, Wickens, M. (1998) Control of translation initiation in animals. In Ann. Rev. Cell Dev. Biol. (James A. Spudich, editor) Annual Reviews, Inc., Palo Alto, CA Vol. 14, pp. 399-458. [PubMed]

Zhang, B, Kraemer, B,SenGupta, D, Fields, S, Wickens, M. (1998) A three-hybrid system to detect and analyze RNA: protein interactions in vivo. In RNA-Protein Interactions: A Practical Approach. (Chris Smith, editor) Oxford University Press, Oxford, England, pp. 195-216.

Gillian-Daniel, DL, Gray, NK, Astrom, J, Barkoff, A, Wickens, M. (1998) Modifications of the 5′ cap of mRNAs during Xenopus oocyte maturation: independence from changes in poly(A) length and impact on translation. Mol. & Cell. Biol. 18, 6152-6163. [PubMed] [PDF]

Coller, JM, Gray, NK, Wickens, M. (1998) mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation. Genes Dev. 12, 3226-3235. [PubMed] [PDF]

Barkoff, A, Ballantyne, S, Wickens, M. (1998) Polyadenylation of multiple mRNAs is required for oocyte maturation. EMBO J. 17, 3168-3175. [PubMed] [PDF]


Jackson, RJ, Wickens, M. (1997) Translational controls impinging on the 5′-untranslated region and initiation factor proteins. In Current Opinion in Genetics & Development (Ron Laskey and Matthew P. Scott eds.) Current Biology Ltd., London, U.K. Vol. 7, pp. 233-241. [PubMed] [PDF]

Wickens, M, Anderson, P, Jackson, RJ. (1997) Life and death in the cytoplasm: messages from the 3’end. In Current Opinion in Genetics & Development (Ron Laskey and Matthew P. Scott eds.) Current Biology Ltd., London, U.K., Vol. 7, pp. 220-232. [PubMed] [PDF]

Zhang, B, Kraemer, B, SenGupta, D, Fields, S, Wickens, M. (1997) A three-hybrid system to detect and analyze RNA-protein interactions in vivo. In The Yeast Two-Hybrid System (P.L. Bartel and Stanley Fields, eds.) Oxford University Press, New York, New York, pp. 298-315.

Puoti, A, Gallegos, M, Zhang, B, Wickens, M, Kimble, J. (1997) Controls of cell fate and pattern by 3′ untranslated regions: The Caenorhabditis elegans sperm/oocyte decision. Cold Spring Harbor Symposia on Quantitative Biology, 62, 19-24. [PubMed]

Zhang, B, Gallegos, M, Puoti, A, Durkin, E, Fields, S, Kimble, J, Wickens, M. (1997) A conserved RNA binding protein that regulates patterning of sexual fates in the C. elegans hermaphrodite germ line. Nature 390, 477-484. [PubMed] [PDF]

Ballantyne, S, Daniel, Jr, D, Wickens, M. (1997) A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation. Mol. Biol. Cell 8, 1633-1648. [PubMed] [PDF]

McCracken, S, Fong, N, Yankulov, K, Ballantyne, S, Pan, G, Greenblatt, J, Patterson, SD, Wickens, M,,Bentley, DL. (1997) The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 385, 357-361. [PubMed] [PDF]


Wickens, M, Belasco, JG, Jacobson, A. (1996) Changes in the length of poly(A) tails and their effects on mRNA translation and turnover. In Nato ASI Series (Orna Resnekov and Alexander von Gabain, eds.) Springer-Verlag, Berlin, Heidelberg, pp. 45-55.

Wickens, M, Kimble, J, Strickland, S. (1996) Translation regulation of developmental decisions. In Translational Control (Michael Mathews, ed.) Cold Spring Harbor Press, New York, pp. 411-450. [PDF]

Verrotti, A, Thompson, S, Wreden, C, Strickland, S, Wickens, M. (1996) Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation. Proc. Natl. Acad. Sci. 93, 9027-9032. [PubMed] [PDF]

SenGupta, D, Zhang, B, Kraemer, B, Prochart, P, Fields, S, Wickens, M. (1996) A three-hybrid system for detecting RNA-protein interactions. Proc. Natl. Acad. Sci. 93, 8496-8501. [PubMed] [PDF]


Sheets, MD, Wu, M, Wickens, M. (1995) Polyadenylation as a control point in Xenopus meiotic maturation. Nature 374, 511-516. [PubMed] [PDF]

Ballantyne, S, Bilger, A, Virtanen, A, Wickens, M. (1995) Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic regulation during oocyte maturation and early development. RNA 1, 64-78. [PubMed] [PDF]

1990 - 1994


Wickens, M, Takayama, K. (1994) Deviants or emissaries. Nature 367, 17-18. [PubMed] [PDF]

Bilger, A, Fox, CA, Wahle, E, Wickens, M. (1994) Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements. Genes Dev. 8, 1106-1116. [PubMed] [PDF]

Sheets, MD, Fox, CA, Hunt, T, Vande Woude, G, Wickens, M. (1994) The 3’UTRs of c-mos and cyclin mRNAs contol translation by regulating cytoplasmic polyadenylation. Genes Dev. 8, 926-938. [PubMed] [PDF]


Wickens, M. (1993) Springtime in the desert. Nature 363, 305-306. [PubMed] [PDF]


Wickens, M. (1992) RNA and the early embryo. Seminars in Developmental Biology 3, 363-366.

Wickens, M. (1992) Forward, backward, how much, when: Mechanisms of poly(A) addition and removal and their role in early development. Seminars in Developmental Biology. 3, 399-412.

Fox, C, Sheets, M, Wahle, E, Wickens, M. (1992) Polyadenylation of maternal mRNA during oocyte maturation: poly(A) addition in vitro requires a regulated RNA binding activity and a poly(A) polymerase. EMBO J. 11, 5021-5032. [PubMed] [PDF]

Forrester, W, Stutz, F, Rosbash, M, Wickens, M. (1992) Defects in mRNA 3′-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure. Genes Dev. 6, 1914-1926. [PubMed] [PDF]


Bardwell, V, Wickens, M, Bienroth, S, Keller, W, Sproat, B, Lamond, A. (1991) Site-directed ribose methylation identifies 2′-OH groups in polyadenylation substrates critical for AAUAAA recognition and poly(A) addition. Cell 65, 125-133. [PubMed] [PDF]

Fleischmann, M, Clark, M, Forrester, M, Wickens, M, Nishimoto, T, Aebi, M. (1991) Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1. Mol. Gen. Gen. 227, 417-423. [PubMed] [PDF]


Wickens, M. (1990) In the beginning is the end: Regulation of poly(A) addition and removal during early development. Trends in Biochem. Sci. 15, 320-324. [PubMed] [PDF]

Wickens, M. (1990) How the messenger got its tail: Addition of poly(A) in the nucleus. Trends in Biochem. Sci. 15, 277-281. [PubMed] [PDF]

Ogg, S, Anderson, A, Wickens, M, (1990) Splicing of a C. elegans myosin pre-mRNA in a human nuclear extract. Nucleic Acids Res. 18, 143-149. [PubMed] [PDF]

Bardwell, V, Zarkower, D, Edmonds, M, Wickens, M. (1990) The enzyme that adds poly(A) to mRNAs is a classical poly(A) polymerase. Mol. Cell Biol. 10, 846-849. [PubMed] [PDF]

Bardwell, V, Wickens, M. (1990) Polyadenylation-specific complexes undergo a transition early during the addition of a polyA tail. Mol. Cell Biol. 10, 295-302. [PubMed] [PDF]

Wigley, P, Sheets, M, Zarkower, D, Whitmer, M, Wickens, M. (1990) Polyadenylation of mRNA: minimal substrates and a requirement for the 2′ hydroxyl of the U in AAUAAA. Mol. Cell Biol. 10, 1705-1713. [PubMed] [PDF]

Bardwell, V, Wickens, M. (1990) Purification of RNA and RNA-protein complexes by R17coat protein affinity chromatography. Nucleic Acids Res. 18, 6587-6594. [PubMed] [PDF]

Sheets, M, Ogg, S, Wickens, M. (1990) Point mutations in AAUAAA and poly(A) addition site: effects on the accuracy and efficiency of cleavage and polyadenylation in vitro. Nucleic Acids Res. 18, 5799-5805. [PubMed] [PDF]

Fox, C, Wickens, M. (1990) Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3′ UTR of certain maternal mRNAs. Genes Dev. 4, 2287-2298. [PubMed] [PDF]

1985 - 1989


Sheets, MD, Wickens, M. (1989) Two phases in the addition of a polyA tail. Genes Dev. 3, 1401-1412. [Abstract] [PDF]

Fox, C, Sheets, MD, Wickens, M. (1989) PolyA tail addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU. Genes Dev. 3, 2151-2162. [PubMed] [PDF]

Conway, L, Wickens, M. (1989) Modification interference, in Methods in Enzymology 180, 369-379. [PDF]


Zarkower, D, Wickens, M. (1988) A functionally redundant downstream sequence is required for mRNA 3′ end formation and for assembly of a pre-cleavage complex in vitro. J. Biol. Chem. 263, 5780-5788. [PubMed] [PDF]


Zarkower, D, Wickens, M. (1987) Formation of mRNA 3′ termini: stability and dissociation of a complex involving the AAUAAA sequence. EMBO J. 6(1), 177-186.  [PubMed] [PDF]

Sheets, MD, Stephenson, P, Wickens, M. (1987) Products of in vitro cleavage and polyadenylation of Simian Virus 40 late pre-mRNAs. Mol. Cell Biol. 7, 1518-1529. [PubMed] [PDF]

Conway, L, Wickens, M. (1987) Analysis of mRNA 3′ end formation by modification interference: the only modifications which prevent processing lie in AAUAAA nd the poly(A) Site. EMBO J. 6, 4177-4184. [PubMed] [PDF]

Zarkower, D, Wickens, M. (1987) Specific pre-Cleavage and post-cleavage complexes involved in the formation of SV40 late mRNA 3′ termini in vitro. EMBO J. 6(13), 4185-4192. [PubMed] [PDF]

Wickens, M, Dahlberg, JE. (1987) RNA-protein interactions. Cell 51, 339-342. [PDF]


Zarkower, D, Stephenson, P, Sheets, MD, Wickens, M. (1986) The AAUAAA sequence is required both for cleavage and for polyadenylation of Simian Virus 40 pre-mRNA in vitro. Mol. Cell Biol. 6, 2317-2323. [PubMed] [PDF]


Conway, L, Wickens, M. (1985) A sequence downstream of AAUAAA is required for formation of simian virus 40 late mRNA 3′ termini in frog oocytes. Proc. Natl. Acad. Sci. 82, 3949-3953. [PubMed] [PDF]

1980 - 1984


Wickens, M, Stephenson, P. (1984) Role of the conserved AAUAAA sequence: four AAUAAA point mutations prevent mRNA 3′ end formation. Science 226, 1045-1051. [PubMed]


Wickens, M, Gurdon, JB. (1983) Post-transcriptional processing of SV40 late transcripts in injected frog oocytes. J. Mol. Biol. 163, 1-26. [Abstract] [PDF]

Gurdon, JB, Wickens, M. (1983) The use of Xenopus oocytes for the expression of cloned genes in Methods in Enzymology, 101, Academic Press, pp. 370-386. [PubMed] [PDF]


Weisbrod, S, Wickens, M, Whytock, S, Gurdon, JB. (1982) Active chromatin of oocytes injected with somatic cell nuclei or cloned DNA. Dev. Biol. 94, 216-229. [PubMed] [PDF]


Wickens, M, Laskey, RA. (1981) Expression of cloned genes in cell-free systems and in microinjected Xenopus oocytes, in Vol. 1, Genetic Engineering, ed. Robert Williamson, Academic Press, London.


Wickens, M, Woo, SL, O’Malley, BW, Gurdon, JB. (1980) Expression of a chicken ovalbumin gene injected into frog oocyte nuclei. Nature 285, 628-634. [PubMed] [PDF]

1975 - 1979


Buell, GN, Wickens, M, Carbon, J, Schimke, RT. (1979) Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs. J. Biol. Chem. 254, 9277-9283. [PubMed] [PDF]

Wickens, M, Buell, GN, Crouse, GF, Carbon, J, Schimke, RT. (1979) Restriction map of the region surrounding the EcoRI site in the pCR1 plasmid and analysis of an inserted ovalbumin Gene 5, 19-43. [Abstract] [PDF]


Wickens, M, Buell, GN, Schimke, RT. (1978) Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid and ovalbumin mRNAs: optimization for full length second strand synthesis by E. coli DNA polymerase I. J. Biol. Chem. 253, 2483-2495. [PubMed] [PDF]

Buell, GN, Wickens, M, Payvar, F, Schimke, RT. (1978) Synthesis of full length cDNAs from four partially purified oviduct mRNAs. J. Biol. Chem. 253, 2471-2482. [PubMed] [PDF]


d’Albis, A, Wickens, M, Gratzer, WB. (1975) Base-pairing of adenine and uracil derivatives in the presence of water. Biopolymers 14, 1423-1435. [PubMed] [PDF]